Spectrophotometric determination of ascorbic acid and dehydroascorbic acid
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چکیده
منابع مشابه
Spectrophotometric determination of ascorbic acid and dehydroascorbic acid.
We present a method for measuring ascorbic acid in methanol/trichloroacetic acid extracts prepared from human plasma after enzymatic oxidation of ascorbic acid to dehydroascorbic acid by ascorbate oxidase. Samples were assayed by spectrophotometrically monitoring the kinetics of the concentration-dependent absorbance changes of dehydroascorbic acid with phosphate-citrate-methanol buffers. Ascor...
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Dehydroascorbic acid is shown to be converted to 2-(threo-l,2,3-trihydroxypropyl)tartronic acid in aqueous alkaline solutions. The structure of the acid was determined by mass spectrometry of its acyclic Me3Si derivative. Mass spectrometric and chromatographic data are compared with those of related compounds. The acid is formed by a benzilic acid rearrangement of the intermediate 2,3-hexodiulo...
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A simple and rapid method has been developed for the determination of ascorbic acid in fruit and pharmaceutical products. The method is based on the measurement of the reaction rate between ammonium molybdate and ascorbic acid in acidic media. The reaction was monitored spectrophotometrically at 800 nm by fixed-time and variable-time methods. The calibration graph was linear in the range of...
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Controlled studies were conducted to find out the plasma values of ascorbic acid, dehydroascorbic acid (DHA), urinary excretion of ascorbic acid and blood levels of glutathione in patients with viral hepatitis, alcoholic hepatitis, cirrhosis of liver and carcinoma of liver. Leucocyte ascorbic acid and DHA/AA index were also determined in order to assess the ascorbic acid status of these patient...
متن کاملkinetic determination of ascorbic acid a with spectrophotometric detection
a simple and rapid method has been developed for the determination of ascorbic acid in fruit and pharmaceutical products. the method is based on the measurement of the reaction rate between ammonium molybdate and ascorbic acid in acidic media. the reaction was monitored spectrophotometrically at 800 nm by fixed-time and variable-time methods. the calibration graph was linear in the range of 5 0...
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ژورنال
عنوان ژورنال: Clinical Chemistry
سال: 1995
ISSN: 0009-9147,1530-8561
DOI: 10.1093/clinchem/41.8.1177